Journal: Journal of aquatic animal health

Article Title: Development of Similar Broth Microdilution Methods to Determine the Antimicrobial Susceptibility of Flavobacterium columnare and F. psychrophilum

doi: 10.1080/08997659.2015.1105878

Figure Lengend Snippet: Flavobacterium columnare and F. psychrophilum isolates used to develop broth microdilution antimicrobial susceptibility testing methods. MSU CVM = Mississippi State University, College of Veterinary Medicine Aquatic Diagnostic Laboratory.

Article Snippet: MSU CVM isolate number Original isolate number Host Year isolated Country of origin Source Serum supplementation Inoculum volume in test broth Cation supplementation Flavobacterium columnare 22650 503–435 Channel Catfish Ictalurus punctatus 2003 USA MSU CVM + + + 22655 503–517 Channel Catfish 2003 USA MSU CVM + + 22683 94–060 Channel Catfish 1994 USA John Hawke b + + + 33677 94–082 Channel Catfish 1994 USA John Hawke + + 33970 ATCC 49512 Brown Trout Salmo trutta 1987 France ATCC + + + 33971 ATCC 49513 Black Bullhead Ameiurus melas 1987 France ATCC + + 36413 CVI unknown Unknown 1991 The Netherlands Olga Haenen c + + 36417 CVI 04017018 Koi a 2004 The Netherlands Olga Haenen + + + 36422 ALG 92–491-C Channel Catfish 1992 USA Joseph Newton d + + + 36434 JIP 07/02 Koi 2002 France Jean-Francois Bernardet e + 36445 Not provided Koi NA USA Hui-Min Hsu f + + Flavobacterium psychrophilum 22645 ATCC 49418T Coho Salmon Oncorhynchus kisutch NA USA ATCC + + + 36391 FLPS 70 Coho Salmon 1981 USA Douglas Call g 36393 FLPS 79 Coho Salmon 1990 USA Douglas Call + + 36394 FLPS 80 Rainbow Trout O. mykiss 1990 USA Douglas Call + + 36395 FLPS 85 Rainbow Trout 1985 USA Douglas Call + + + 36396 FLPS 96 Ayu Plecoglossus altivelis altivelis 1988 Japan Douglas Call + 36400 FLPS 69 Coho Salmon 1981 USA Douglas Call + + + 36403 FLPS 74 Coho Salmon 1989 USA Douglas Call 36408 FLPS 99 Coho Salmon 1991 USA Douglas Call + + 36410 AU0205 Atlantic Salmon Salmo salar 2006 Chile Ruben Avendaño-Herrera h + + + 36411 AU2706 Rainbow Trout 2006 Chile Ruben Avendaño-Herrera + + 36429 LVDI 5/I Common Carp 1992 France Jean-Francois Bernardet + + + 36430 LVDJ XP189 Tench Tinca tinca 1992 France Jean-Francois Bernardet + + Open in a separate window a A variant of Common Carp Cyprinus carpio . b Louisiana State University, School of Veterinary Medicine Aquatic Diagnostic Laboratory. c Wageningen University and Research Centre, Central Veterinary Institute. d Auburn University, College of Veterinary Medicine. e Institut National de la Recherche Agronomique, Unite de Virologie et Immunologie Moleculaires. f University of Wisconsin–Madison, Veterinary Diagnostic Laboratory. g Washington State University, College of Veterinary Medicine Animal Disease Diagnostic Laboratory. h Universidad Andres Bello Interdisciplinary Center for Aquaculture Research.

Techniques: Diagnostic Assay, Isolation

Journal: PLoS Pathogens

Article Title: Inhibition of caspase-1 or gasdermin-D enable caspase-8 activation in the Naip5/NLRC4/ASC inflammasome

doi: 10.1371/journal.ppat.1006502

Figure Lengend Snippet: (A-D) Bone marrow-derived macrophages (BMDMs) from C57BL/6 (open circles), Nlrc4 -/- (closed squares), Casp1/11 -/- (closed triangles) and Asc/Casp1/11 -/- (open triangles) mice were infected with motility-deficient mutants expressing flagellin ( fliI - , A), with flagellin-deficient mutants ( flaA - , B), L . gratiana (C) or with L . micdadei (D) at a MOI of 10. The cells were incubated for 24, 48 and 72 hours for CFU determination. Data show the average ± SD of triplicate wells. * , P <0.05 compared with Casp-1/11 -/- BMDMs. # , P <0.05 compared with C57BL/6 BMDMs, ANOVA. (E-I) BMDMs from Casp1/11 -/- and Asc/Casp1/11 -/- mice were transduced with a retrovirus encoding shRNA sequences to target caspase-8 (Seq1, Seq2) and a non-target shRNA sequence (NT). (E) Caspase-8 silencing was confirmed by western blot analysis. Cell lysates were separated by SDS-PAGE, blotted and probed with anti-caspase-8 (pro-caspase-8 p55) and anti-α-actin. (F-I) Transduced Casp1/11 -/- (F, H) and Asc/Casp1/11 -/- (G, I) BMDMs were infected with fliI - (F, G) or flaA - (H, I) at a MOI of 10 and incubated for 24, 48 and 72 hours for CFU determination. Data show the average ± SD of triplicate wells. * , P <0.05 compared with NT shRNA, ANOVA. NT, non-target shRNA. Data are presented for one representative experiment of four (A), two (B-D) and one (F-I) experiments performed with similar results.

Article Snippet: L . micdadei (ATCC 33218) and L . gratiana (ATCC 49413) were used to generate streptomycin-resistant strains.

Techniques: Derivative Assay, Infection, Expressing, Incubation, Transduction, shRNA, Sequencing, Western Blot, SDS Page

Journal: PLoS Pathogens

Article Title: Inhibition of caspase-1 or gasdermin-D enable caspase-8 activation in the Naip5/NLRC4/ASC inflammasome

doi: 10.1371/journal.ppat.1006502

Figure Lengend Snippet: C57BL/6 (open circles), Nlrc4 -/- (closed squares), Casp1/11 -/- (closed triangles) and Asc/Casp1/11 -/- (open triangles) mice were infected with motility-deficient L . pneumophila mutants expressing flagellin ( fliI - , A, D), with flagellin-deficient L . pneumophila ( flaA - , D), with L . gratiana (B) or with L . micdadei (C) at a dose of 10 5 bacteria/mice. The mice were euthanized at 4, 48 or 72 hours after infection. Dilutions of the lung homogenates were added to charcoal-yeast extract agar plates for colony-forming unit determination. Each dot represents a single animal, and the horizontal lines represent the averages. * , P <0.05. # , indicates P <0.05 compared with C57BL/6, Mann Whitney test. Data are presented for one representative experiment of five (A), one (B-C) and two (D) experiments performed with similar results.

Article Snippet: L . micdadei (ATCC 33218) and L . gratiana (ATCC 49413) were used to generate streptomycin-resistant strains.

Techniques: Infection, Expressing, Bacteria, MANN-WHITNEY